Faculty Research 1980 - 1989

Title

Genetic regulation of sn-glycerol-3-phosphate dehydrogenase in brown adipose tissue.

Document Type

Article

Publication Date

1986

Keywords

Brown-Fat: en, Genes-Regulator, Genes-Structural, Glycerolphosphate-Dehydrogenase: bi, ge, Mice, Mice-Inbred-Strains, Nucleic-Acid-Hybridization, Organ-Specificity, RNA: ip, RNA-Messenger: ge, Species-Specificity, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Location

1,314.

JAX Source

Genetics. 1986 Oct; 114(2):567-78.

Grant

HD08431

Abstract

The levels of sn-glycerol-3-phosphate dehydrogenase (GPDH) were determined in the brown adipose tissue (BAT) of different inbred strains of mice. The BAT of the BALB/cJ strain contains twice as much enzyme activity per milligram protein as do other strains. The appearance of this difference is developmentally dependent, since it is not detected in BAT until 25-30 days postpartum. Genetic analysis of this strain difference has shown that the mechanism of inheritance involves at least two genes, one of which is linked to the Gdc-1 structural locus on chromosome 15. Determinations of GPDH synthesis by immunoprecipitation of GPDH protein labeled in vivo with [3H]leucine, and of GPDH mRNA by Northern blot analysis, establish that in BALB/cJ mice higher rates of enzyme synthesis are determined by elevated levels of GPDH mRNA. It was also found that cold stress increases GPDH mRNA levels in all the strains examined.

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