Faculty Research 1980 - 1989

Title

Distinction of B cell maturation factors from lymphokines affecting B cell growth and viability.

Document Type

Article

Publication Date

1986

Keywords

Antibodies-Monoclonal: ph, B-Lymphocytes: im, cy, Binding-Competitive, Cell-Survival, Cell-Free-System, Chromatography-Affinity, Chromatography-Agarose, Growth-Substances: cl, ip, Interferon-Type-II: im, Isoelectric-Focusing, Lymphocyte-Transformation, Lymphokines: cl, ip, ph, Mice, Mice-Inbred-C57BL, Molecular-Weight, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: me

JAX Source

J-Immunol. 1986 Feb 1; 136(3):994-8.

Grant

CA35845, AI20232

Abstract

Supernatants from S26.5 helper T cells, autoimmune viable motheaten (mev/mev) mouse spleen cells, EL4 lymphoma cells, and recombinant DNA-derived interferon gamma (IFN-gamma), all of which display B cell maturation factor (BMF) activity, were assayed for effects on B cell proliferation alone and with Dextran Sulfate (DxS) and anti-immunoglobulin antibodies (alpha-Ig). Both EL4 and S26.5 supernatants showed BCGF-II (DxS co-stimulator) activity, whereas only EL4 supernatant had BCGF-I (alpha-Ig co-stimulator or BSF-I) activity. Supernatants from mev/mev spleen cells and recombinant DNA-derived IFN-gamma showed no activity in either assay. Fractionation of S26.5 supernatant by chromatofocusing showed a divergence of BMF activity (BMF-T, pIa of 6.0) from BCGF-II activity (pIa of 5.4), providing evidence for their physical nonidentity. IFN-gamma, which decreases B cell viability in culture, was separable from BMF-T by phenyl-Sepharose chromatography. BMF-T from S26.5 supernatant was separated from IFN-gamma and BCGF-II and was shown to induce B cell maturation without affecting B cell proliferation. The molecular characteristics of the purified BMF-T were pIa 6.0, Mr 55,000 by G-75 gel filtration, and Mr 16,000 by SDS-PAGE. These data demonstrate that several lymphokines (BMF) exist that mediate the maturation of B cells to active Ig secretion without stimulating B cell proliferation.

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