Faculty Research 1980 - 1989
Stability of retrovirally transduced markers in a rat cell line.
Document Type
Article
Publication Date
1986
Keywords
Cell-Line, Genes-Structural, Genes-Viral, Genetic-Vectors, Herpesvirus-Hominis: ge, Hypoxanthine-Phosphoribosyltransferase: ge, Moloney-Leukemia-Virus: ge, Plasmids, Rats, Thymidine-Kinase: ge, Transduction-Genetic, Transfection
First Page
575
Last Page
583
JAX Source
Somatic-Cell-Mol-Genet. 1986 Nov; 12(6):575-83.
Abstract
A MoMLV-based retroviral vector capable of transmitting and expressing both the human hypoxanthine phosphoribosyltransferase (hprt) coding sequence and the Herpes simplex type 1 thymidine kinase (tk) gene has been constructed. After infection of a rat cell line, cell clones were selected on the basis of expressing both markers. They were subsequently found to contain a single provirus of the expected topology. The ease with which loss of expression of the markers can be monitored has allowed us to make observations on the stability of proviral genes. In particular, we have found indirect evidence of strong position effects on proviral gene expression by comparing the characteristic frequency of marker loss in different clonal proviral lines. Effects of the selection protocol on the apparent frequency of variants have also been noted. Finally, a combination of molecular and genetic observations lead us to invoke chromosome loss as the major factor influencing marker stability in this system.
Recommended Citation
Stone JC,
Dower NA,
Siminovitch L.
Stability of retrovirally transduced markers in a rat cell line. Somatic-Cell-Mol-Genet. 1986 Nov; 12(6):575-83.