Faculty Research 1980 - 1989


Early replication and expression of oocyte-type 5S RNA genes in a Xenopus somatic cell line carrying a translocation.

Document Type


Publication Date



Cell-Division, Cell-Line, Female, Gene-Expression-Regulation, Interphase, Models-Genetic, Oocytes, RNA-Ribosomal: bi, ge, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Transcription-Factors: bi, ge, Translocation-(Genetics), Xenopus-laevis: ge, me

JAX Source

Proc Natl Acad Sci U S A 1986 Jul;83(14):5150-4


GM23241, GM09947, CA09302


In Xenopus somatic cells, the somatic-type 5S RNA genes replicate early in S phase, bind the transcription factor TFIIIA, and are expressed; in contrast, the late replicating oocyte-type genes do not bind TFIIIA and are transcriptionally inactive. These facts support a model in which the order of replication of the somatic-type versus the oocyte-type 5S genes causes their differential expression in somatic cells due to sequestration of TFIIIA by the early-replicating somatic genes. Here we provide further evidence for the model by showing that in one Xenopus cell line in which some oocyte-type 5S genes are translocated, some oocyte-type 5S genes replicate early and are expressed.

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