Transgenesis by means of blastocyst-derived embryonic stem cell lines.
Blastocyst: ph, Cell-Differentiation, Chimera, Drug-Resistance, Germ-Cells, Mice: ge, Pedigree, Phosphotransferases: ge, Skin-Pigmentation, Spleen: cy, Stem-Cells: ph, SUPPORT-NON-U-S-GOVT, Transfection
Proc Natl Acad Sci U S A 1986 Dec;83(23):9065-9
This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.
Gossler, A; Doetschman, T; Korn, R; Serfling, E; and Kemler, R, " Transgenesis by means of blastocyst-derived embryonic stem cell lines." (1986). Faculty Research 1980 - 1989. 841.
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