Faculty Research 1980 - 1989

Title

An improved method for preparing G-banded chromosomes from mouse peripheral blood.

Document Type

Article

Publication Date

1987

Keywords

Chromosome-Banding: mt, Comparative-Study, Female, Karyotyping: mt, Lipopolysaccharides, Lymphocytes: ul, Mice, Mice-Inbred-C57BL, Mice-Inbred-DBA, Mitogens, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Cytogenet-Cell-Genet. 1987; 45(2):70-4.

Grant

HD17784, HD52917

Abstract

We describe here several improvements in the method we originally developed to prepare mitotic chromosomes from peripheral blood of laboratory mice. In addition, we have tried several methods to improve metaphase yield from lymphocytes of the inbred strain DBA/2J, which respond poorly to phytohemagglutinin. The yield of mitoses from DBA/2J cells cannot be improved by enhancing T-cell response using interleukin-2 or by using a different T-cell mitogen, concanavalin A. Metaphase yield from peripheral blood cells of DBA/2J mice can be improved significantly by adding lipopolysaccharide to cultures, probably stimulating B-cell as well as T-cell proliferation.

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