Faculty Research 1980 - 1989


Genetic variations in kinetic constants that describe beta-glucuronidase mRNA induction in androgen-treated mice.

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Female, Glucuronidase: bi, ge, Haplotypes, Kidney: de, me, Kinetics, Mice, Mice-Inbred-Strains, RNA-Messenger: bi, ge, SUPPORT-U-S-GOVT-P-H-S, Testosterone, Transcription-Genetic, Variation-(Genetics)

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Mol Cell Biol 1987 Mar; 7(3):1085-90.




The kinetics of beta-glucuronidase mRNA induction by androgen in mouse kidney were determined for A, B, and CS haplotypes of the beta-glucuronidase gene. After a lag period, the kinetics of mRNA (R) induction are approximated by the turnover equation dR/dt = k1 - k2R. The A haplotype differs from the B primarily in the duration of the lag period and in k1, the rate constant determining the initial slope of the induction curve. The CS haplotype differs from B primarily in k2, the first-order rate constant that determines the half-time for induction. None of the haplotypes differs significantly in the half-life of beta-glucuronidase mRNA as measured by deinduction. Thus, there was no correlation between the half-time or extent of induction and the half-life of the RNA. Comparing half-times for induction with the half-life of the mRNA suggests that message stabilization can at most account for only part of the induction. We conclude that transcriptional activation of the beta-glucuronidase gene must be an important component of induction. Estimating absolute numbers of mRNA molecules and absolute rates of gene transcription, it appears that before induction there is approximately one molecule of beta-glucuronidase mRNA per cell and that each gene copy is transcribed once every 35 to 40 h. Depending on the haplotype examined, after induction, mRNA goes up to 80 to 400 molecules per induced cell.(ABSTRACT TRUNCATED AT 250 WORDS)

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