Faculty Research 1990 - 1999
Molecular cloning of the t complex responder genetic locus.
Document Type
Article
Publication Date
1990
Keywords
Blotting-Southern, Cloning-Molecular, Cosmids, Genes-Reiterated, Haplotypes, Male, Mice, Nuclear-Proteins: ge, Recombination-Genetic, Restriction-Fragment-Length-Polymorphisms, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Testis: me
First Page
134
Last Page
140
JAX Source
Genomics 1990 Sep;8(1):134-40
Grant
R01HD24374-02
Abstract
Although mouse t haplotypes carry recessive mutations causing male sterility and embryonic lethality, they persist in wild mouse populations via male transmission ratio distortion (TRD). Genetic evidence suggests that at least five t-haplotype-encoded loci combine to cause TRD. One of these loci, called the t complex responder (Tcr), is absolutely required for any deviation from Mendelian segregation to occur. A candidate for the Tcr gene has previously been identified. Evidence that this gene represents Tcr is its localization to the appropriate genomic subregion and testis-specific expression pattern. Here, we report the molecular cloning of the region between recombinant chromosome breakpoints defining the Tcr locus. These results circumscribe Tcr to a 150- to 220-kb region of DNA, including the 22-kb candidate responder gene. This gene and two other homologs were created by large genomic duplications, each involving segments of DNA 10-fold larger than the individual genes.
Recommended Citation
Rosen LL,
Bullard DC,
Silver LM,
Schimenti JC.
Molecular cloning of the t complex responder genetic locus. Genomics 1990 Sep;8(1):134-40