Faculty Research 1990 - 1999


Molecular cloning of the t complex responder genetic locus.

Document Type


Publication Date



Blotting-Southern, Cloning-Molecular, Cosmids, Genes-Reiterated, Haplotypes, Male, Mice, Nuclear-Proteins: ge, Recombination-Genetic, Restriction-Fragment-Length-Polymorphisms, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Testis: me

JAX Source

Genomics 1990 Sep;8(1):134-40




Although mouse t haplotypes carry recessive mutations causing male sterility and embryonic lethality, they persist in wild mouse populations via male transmission ratio distortion (TRD). Genetic evidence suggests that at least five t-haplotype-encoded loci combine to cause TRD. One of these loci, called the t complex responder (Tcr), is absolutely required for any deviation from Mendelian segregation to occur. A candidate for the Tcr gene has previously been identified. Evidence that this gene represents Tcr is its localization to the appropriate genomic subregion and testis-specific expression pattern. Here, we report the molecular cloning of the region between recombinant chromosome breakpoints defining the Tcr locus. These results circumscribe Tcr to a 150- to 220-kb region of DNA, including the 22-kb candidate responder gene. This gene and two other homologs were created by large genomic duplications, each involving segments of DNA 10-fold larger than the individual genes.

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