Faculty Research 1990 - 1999

Title

Characterization of the mouse Prox1 gene.

Document Type

Article

Publication Date

1998

Keywords

Animal, Base-Sequence, Chromosome-Mapping, Cloning-Molecular, Conserved-Sequence, DNA-Complementary, Exons, Genes-Homeobox, Homeodomain-Proteins: bi, ge, Human, Introns, Lens-Crystalline: me, Mice, Mice-Mutant-Strains, Molecular-Sequence-Data, Recombinant-Proteins: bi, Retina: me, RNA-Messenger: bi, Sequence-Alignment, Sequence-Homology-Nucleic-Acid, SUPPORT-U-S-GOVT-P-H-S

First Page

684

Last Page

689

JAX Source

Biochem Biophys Res Commun 1998 Jul 30;248(3):684-9

Grant

EY07757/EY/NEI, EY05578/EY/NEI

Abstract

Prox1, a vertebrate homologue of Drosophila prospero, encodes a divergent homeodomain protein. We have isolated and characterized full length mouse Prox1 cDNA and genomic clones. Mouse Prox1 gene mapped to position 106.3 cM from the centromere of Chromosome 1, which is very close to the retinal degeneration mutation, rd3. Although the coding sequence and exon-intron junctions of the Prox1 genes of wild type and rd3 mutant mice are identical, Northern blot analysis indicated that the ratio of the short (2.3 kb) and long (8 kb) forms of Prox1 mRNA is different in RNA isolated from wild type and rd3 retinas. Immunostaining of the eyes from wild type and rd3 animals also revealed differences in the distribution of Prox1 protein in the retina and lens. These data suggest that the rd3 mutation affects expression of the mouse Prox1 gene.

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