
Faculty Research 1990 - 1999
Title
Characterization of the mouse Prox1 gene.
Document Type
Article
Publication Date
1998
Keywords
Animal, Base-Sequence, Chromosome-Mapping, Cloning-Molecular, Conserved-Sequence, DNA-Complementary, Exons, Genes-Homeobox, Homeodomain-Proteins: bi, ge, Human, Introns, Lens-Crystalline: me, Mice, Mice-Mutant-Strains, Molecular-Sequence-Data, Recombinant-Proteins: bi, Retina: me, RNA-Messenger: bi, Sequence-Alignment, Sequence-Homology-Nucleic-Acid, SUPPORT-U-S-GOVT-P-H-S
First Page
684
Last Page
689
JAX Source
Biochem Biophys Res Commun 1998 Jul 30;248(3):684-9
Grant
EY07757/EY/NEI, EY05578/EY/NEI
Abstract
Prox1, a vertebrate homologue of Drosophila prospero, encodes a divergent homeodomain protein. We have isolated and characterized full length mouse Prox1 cDNA and genomic clones. Mouse Prox1 gene mapped to position 106.3 cM from the centromere of Chromosome 1, which is very close to the retinal degeneration mutation, rd3. Although the coding sequence and exon-intron junctions of the Prox1 genes of wild type and rd3 mutant mice are identical, Northern blot analysis indicated that the ratio of the short (2.3 kb) and long (8 kb) forms of Prox1 mRNA is different in RNA isolated from wild type and rd3 retinas. Immunostaining of the eyes from wild type and rd3 animals also revealed differences in the distribution of Prox1 protein in the retina and lens. These data suggest that the rd3 mutation affects expression of the mouse Prox1 gene.
Recommended Citation
Tomarev SI,
Zinovieva RD,
Chang B,
Hawes NL.
Characterization of the mouse Prox1 gene. Biochem Biophys Res Commun 1998 Jul 30;248(3):684-9