Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes.
Animal, Base-Sequence, Chimera, Cloning-Molecular, DNA-Primers, Exons, Gene-Targeting, Genetic-Vectors, Genomic-Library, Mammals, Mice, Mice-Inbred-Strains, Molecular-Sequence-Data, Mutagenesis, Polycystic-Kidney-Diseases, Polymerase-Chain-Reaction, Recombination-Genetic, Saccharomyces-cerevisiae, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Transformation-Genetic
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Mutat Res 1998 Jun; 401(1-2):11-25.
We have developed a new procedure utilizing microhomologous recombination in yeast to generate targeting constructs for producing targeted mutations in mice. This procedure is rapid and efficient, and should be directly applicable to all mammalian genes. Moreover, only minimal information about the locus being targeted is required. The feasibility of this approach was demonstrated by producing another allele of the mouse Tg737 polycystic kidney gene.
Khrebtukova, I; Michaud, E J.; Foster, C M.; Stark, K L.; Garfinkel, D J.; and Woychik, R P., " Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes." (1998). Faculty Research 1990 - 1999. 1200.