Faculty Research 1990 - 1999

Biochemical and immunogenetic analysis of an immunodominant peptide (B6dom1) encoded by the classical H7 minor histocompatibility locus.

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Cell-Line, Chromosome-Mapping, Clone-Cells, Cytotoxicity-Tests-Immunologic, Cytotoxicity-Immunologic: ge, Female, Immunodominant-Epitopes: ge, im, Male, Mice, Mice-Congenic, Mice-Inbred-C3H, Mice-Inbred-C57BL, Minor-Histocompatibility-Antigens: ge, Minor-Histocompatibility-Loci: ge, im, Oligopeptides: ge, im, Organ-Specificity: im, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes-Cytotoxic: im

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J Immunol 1999 Apr 15;162(8):4502-10




Of the many minor histocompatibility (H) Ags that have been detected in mice, the ability to induce graft vs host disease (GVHD) after bone marrow transplantation is restricted to a limited number of immunodominant Ags. One such murine Ag, B6dom1, is presented by the H2-Db MHC class I molecule. We present biochemical evidence that the natural B6dom1 peptide is indistinguishable from AAPDNRETF, and we show that this peptide can be isolated from a wide array of tissues, with highest levels from the lymphoid organs and lung. Moreover, we employ a novel, somatic cell selection technique involving CTL-mediated immunoselection coupled with classical genetics, to show that B6dom1 is encoded by the H7 minor H locus originally discovered approximately 40 years ago. These studies provide a molecular genetic framework for understanding B6dom1, and exemplify the fact that mouse minor H loci that encode immunodominant CTL epitopes can correspond to classical H loci originally identified by their ability to confer strong resistance to tumor transplantation. Additionally, these studies demonstrate the utility of somatic cell selection approaches toward resolving H Ag immunogenetics.

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