Faculty Research 1990 - 1999

Telomere-related markers for the pseudoautosomal region of the mouse genome.

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Base-Sequence, Blotting-Southern, Crosses-Genetic, DNA: ge, DNA-Probes, Female, Genetic-Markers, Heterochromatin: ph, Linkage-(Genetics), Male, Mice, Mice-Inbred-Strains: ge, Muridae: ge, Oigonucleotide-Probes, Recombination-Genetic, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Telomere: ph, X-Chromosome, Y-Chromosome

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Proc Natl Acad Sci U S A 1992 Mar 15;89(6):2160-4


GM20919, CA09217


The pseudoautosomal (PA) region of the mammalian genome is the region of the X and Y chromosomes that shares extensive DNA sequence homology and is of special interest because it may play an essential role during male meiosis. We have identified three telomere-related restriction fragments from the PA region of the mouse genome, using an oligonucleotide probe composed of the mammalian telomere consensus sequence TTAGGG. PA assignment of two C57BL/6J-derived fragments was initially suggested by analysis of DNAs from progeny sired by C57BL/6J males carrying the rearranged Y chromosome, Y*: the hybridization intensity of both fragments was concordant with the sex-chromosome complement of the offspring. Further analysis indicated that both fragments were present in female and male F1, mice regardless of the sex of their C57BL/6J parent--a criterion for autosomal or PA linkage. Both fragments were closely linked to each other and located on the X chromosome distal to amelogenin (Amg)--in agreement with X or PA linkage. Confirmation of the PA derivation of these fragments was accomplished by following their segregation in a cross involving XY* males mated to DBA/2J females. A similar experiment identified a third PA-derived restriction fragment of LT/SvEi origin. Identification of PA-derived telomere-related restriction fragments will enable further genetic analysis of this region of the mouse genome.

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