Faculty Research 1990 - 1999

Mls-1 is encoded by the long terminal repeat open reading frame of the mouse mammary tumor provirus Mtv-7.

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Animal, B-Lymphocytes: im, Base-Sequence, Cell-Line, Cloning-Molecular, Comparative-Study, Mammay-Cancer-Virus: ge, Mice, Mice-Inbred-Strains, Minor-Lymphocyte-Stimulatory-Antigens: ge, Molecular-Sequence-Data, Oligodeoxyribonucleotides, Open-Reading-Frames, Proviruses: ge, Receptors-Antigen-T-Cell: ge, Repetitive-Sequences-Nucleic-Acid, Restriction-Mapping, Sequence-Homology-Nucleic-Acid, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: im, Transfection

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Proc Natl Acad Sci U S A 1992 Jun 15;89(12):5432-6


The murine Mls-1 antigen is the prototype of endogenous superantigens, molecules whose activities lead to deletion of T cells expressing certain T-cell receptor V beta genes from the mature repertoire. However, Mls-1 also stimulates T cells expressing these particular V beta genes (V beta 6, V beta 7, V beta 8.1, and V beta 9) in vitro, making it one of the strongest known T-cell activators. We have recently reported that the Mls-1 gene is closely linked to the endogenous mammary tumor virus Mtv-7. We now demonstrate that Mls-1 is encoded by the open reading frame in the U3 region of the long terminal repeat of Mtv-7. However, control of expression of this molecule seems complex, depending on the promoter used for the transfection experiments. The sequence of the Mtv-7 open reading frame differs from all other known mammary tumor virus open reading frame sequences in the 3' end, suggesting that the T-cell receptor V beta specificity is conferred by the C terminus of the molecule. The predicted structure of the protein encoded by the open reading frame is consistent with a type II transmembrane molecule where the C terminus is extracellular.

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