Mapping of ornithine aminotransferase gene sequences to mouse chromosomes 7, X, and 3.
Animal, Chromosome-Mapping, Cricetulus, Crosses-Genetic, DNA-Restriction-Enzymes, Female, Hamsters, Hybrid-Cells, Male, Mice, Mice-Inbred-Strains, Ornithine-Aminotransferase: ge, Recombination-Genetic: ge, Restriction-Fragment-Length-Polymorphisms, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
Mamm Genome 1992;3(1):17-22
EY05633, NS05096, HG00169, +
Ornithine aminotransferase (OAT), a mitochondrial matrix enzyme, is deficient in patients with gyrate atrophy of the choroid and retina. In human, the OAT structural gene maps to Chromosome (Chr) 10q26 and several OAT-related sequences, some of which are known to be processed pseudogenes, which map to Xp11.3-11.21. Here, we report chromosomal localization in the mouse of the OAT gene and related sequences. Genomic DNA blot analysis of a well-characterized panel of Chinese hamster x mouse somatic cell hybrids using a human OAT probe revealed two murine loci, one on mouse Chr 7 and the other on Chr X. In addition, segregation of restriction fragment length polymorphisms (RFLPs) detected by the OAT probe in recombinant inbred (RI) strains detected a third locus on Chr 3 and positioned the X locus near Cf-8 and Rsvp. Progeny of an intersubspecific backcross were used to map the Chr 7 locus between Tyr and Int-2, near Cyp2e-1.
Ramesh, V; Cheng, S V.; Kozak, C A.; Herron, B J.; Shih, V E.; Taylor, B A.; and Gusella, J F., " Mapping of ornithine aminotransferase gene sequences to mouse chromosomes 7, X, and 3." (1992). Faculty Research 1990 - 1999. 269.