Faculty Research 1990 - 1999


Complex patterns of sequence variation and multiple 5' and 3' ends are found among transcripts of the erythroid ankyrin gene.

Document Type


Publication Date



Animal, Ankyrins: ge, Base-Sequence, Cerebellum: ph, Cloning-Molecular, Comparative-Study, DNA: ge, DNA-Probes, Exons, Gene-Library, Mice, Mice-Inbred-C57BL, Molecular-Sequence-Data, Organ-Specificity, Polymerase-Chain-Reaction: mt, Restriction-Mapping, Reticulocytes: ph, Sequence-Homology-Amino-Acid, Spleen: ph, SUPPORT-U-S-GOVT-P-H-S, Transcription-Genetic, Variation-(Genetics)

JAX Source

J Biol Chem 1993 May 5;268(13):9533-40




The structural protein ankyrin functions in red blood cells to link the spectrin-based membrane skeleton to the plasma membrane. Ankyrin proteins are now known to occur in most cell types, and two distinct ankyrin genes have been identified (erythroid (Ank-1) and brain (Ank-2)). We have characterized transcripts of the mouse erythroid ankyrin gene by cDNA cloning and DNA sequencing. Ank-1 transcripts of 7.5 and 9.0 kilobases are found in erythroid tissues, and a 9.0-kilobase transcript is found in cerebellum. RNA hybridization blot analysis of 13 additional mouse tissues has detected four novel Ank-1 transcripts (5.0, 3.5, 2.0, and 1.6 kilobases in size). Sequencing of Ank-1 cDNA clones isolated from mouse reticulocyte, spleen, and cerebellar libraries has identified (i) multiple 5' ends that indicate possible multiple promoters; (ii) alternative polyadenylation sites that probably account for the 7.5- and 9.0-kilobase size difference; (iii) a variety of small insertions and deletions that could produce transcripts (and ultimately proteins) of nearly identical size, but different functions; and (iv) clones with large deletions of coding sequence that account for the smaller transcripts seen in spleen, skeletal muscle, and heart.