Faculty Research 1990 - 1999

Analysis of the Pax-3 gene in the mouse mutant splotch.

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Alternative-Splicing, Animal, Base-Sequence, Chromosome-Mapping, Crosses-Genetic, Female, Gene-Deletion, Heterozygote, Homozygote, Linkage-(Genetics), Male, Mice, Mice-Inbred-C3H: ge, Mice-Neurologic-Mutants: ge, Molecular-Sequence-Data, Mutation, Oligodeoxyribonucleotides, Phenotype, Polymerase-Chain-Reaction, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Transcription-Genetic

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Genomics 1993 Aug;17(2):355-63


HD25389/HD/NICHD, NO1-CO-74101/CO/NCI


In a linkage analysis of Pax-3 and splotch no recombinations were found in 117 backcross mice. Molecular analysis of Pax-3 in three alleles of splotch shows a number of significant alterations to the Pax-3 gene. In Sp/Sp embryos, cDNA PCR analysis reveals a shortened transcript in which exon 4 of Pax-3 is deleted due to mutation of the splice acceptor site of intron 3. In the Sp4H allele, the Pax-3 gene is deleted and in Spd embryos, Pax-3 expression is significantly lower than that in normal littermate embryos. The linkage analysis, shortened Pax-3 transcript in Sp, and deletion of Pax-3 in Sp4H described here, together with the previous report of an intragenic deletion in Pax-3 in Sp2H mice and the deletion of Pax-3 in Spr mice, provide strong evidence for the allelic identity of Pax-3 and Sp.