Faculty Research 1990 - 1999


Complexity at the mouse minor histocompatibility locus H-4.

Document Type


Publication Date



Genes-Immune-Response, Helper-Cells: im, In-Vitro, Lymphocyte-Transformation, Major-Histocompatibility-Complex, Mice: ge, im, Minor-Histocompatibility-Loci: ge, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: im, T-Lymphocytes-Cytotoxic: im

JAX Location


JAX Source

Immunogenetics 1990; 31(1):7-12.


AI24544, 2


The fine immunogenetics of the chromosome 7 mouse minor histocompatibility (H) locus H-4 was investigated. Both class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) and class II MHC-restricted "helper: T cells (TH) specifically reactive with H-4 antigens were isolated as clones and were used as genetic probes for classical backcross segregation analysis. Results of a four point cross indicated that the H-4 locus was actually comprised of two genes, that have been designated H-46 and H-47. The former encodes antigens recognized by the TH and the latter encodes antigens recognized by the CTL. Moreover, these two genes could be separated from the gene pink-eyed dilution (p) which was found to be "sandwiched: between them. The functional significance of a minor H congenic strain differing by both TH-defined H-46 and CTL-defined H-47 was addressed using F1 complementation tests. Such studies indicated that immune responses against H-46 antigens was required for generation of H-47-specific CTL. Altogether, these results suggest selective presentation of different minor H gene products by class I or class II MHC proteins and that the minor H "locus: H-4 may have necessarily included both TH and CTL-defined genes because of requisite TH-CTL collaboration.

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