Faculty Research 1990 - 1999

Spontaneous, heritable colitis in a new substrain of C3H/HeJ mice.

Document Type


Publication Date



Breeding, Cecum: pa, Colitis: ge, mi, pa, Colon: pa, Disease-Models-Animal, Disease-Susceptibility: ge, Female, Inflammatory-Bowel-Diseases: ge, mi, pa, Male, Mice, Mice-Inbred-C3H, Pedigree, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

First Page


Last Page


JAX Source

Gastroenterology 1994 Dec;107(6):1726-35




BACKGROUND/AIMS: C3H/HeJ mice at the Jackson Laboratory have periodically been culled because of the occurrence of soft feces, perianal ulceration, and right-sided colitis. No pathogens have been isolated. The goal of the current study was to establish a substrain with a high incidence of this disease. METHODS: Affected male and female C3H/HeJ mice were bred. The clinical, pathological, microbiological, and genetic features of 216 mice of the resulting pedigree were characterized. RESULTS: A severely affected female crossed with a normal male resulted in a new substrain, denoted C3H/HeJBir, with a high incidence of right-sided colitis. Histologically, lesions occurred primarily in the cecum and proximal colon, characterized by acute and chronic inflammation, crypt abscesses, ulcerations, regenerative hyperplasia, and submucosal scarring. Such colitis peaked at 3-6 weeks; however, similar disease was found sporadically in animals more than 1 year of age. Small lesions at the anorectal junction were common throughout life. An extensive search fo r pathogens was negative. Genetic analysis of C3H/HeJBir mice suggested that the disease was inherited as a quanti tative trait. CONCLUSIONS: C3H/HeJBir mice develop a spontaneous, heritable form of idiopathic inflammatory bowel disease and will be a valuable resource for genetic and immunologic studies of this disease.

Please contact the Joan Staats Library for information regarding this document.