Faculty Research 1990 - 1999
Genomic organization, alternative polyadenylation, and chromosomal localization of Grg, a mouse gene related to the groucho transcript of the Drosophila Enhancer of split complex.
Document Type
Article
Publication Date
1994
First Page
194
Last Page
201
JAX Source
Genomics 1994 May 1;21(1):194-201
Grant
NO1-CO-74101/CO/NCI
Abstract
The Grg gene encodes a 197-amino-acid protein homologous to the amino-terminal domain of the product of the groucho gene of the Drosophila Enhancer of split complex. We describe here the genomic organization of the mouse Grg gene. It spans approximately 7 kb on chromosome 10 and consists of seven exons. The 3' region of the Grg gene contains two functional polyadenylation sites that give rise to two transcripts that are differentially expressed among adult mouse tissues. The promoter region is very GC rich and lacks TATA box and initiator sequences. Primer extension analysis and ribonuclease protection assays show that Grg has a major transcription start site situated down-stream of putative binding motifs for the transcription factors Sp1, E2A, and PuF.
Recommended Citation
Mallo M,
Steingrimsson E,
Copeland NG,
Jenkins NA,
Gridley T.
Genomic organization, alternative polyadenylation, and chromosomal localization of Grg, a mouse gene related to the groucho transcript of the Drosophila Enhancer of split complex. Genomics 1994 May 1;21(1):194-201