The therapeutic efficacy of murine anti-tumor T cells: freshly isolated T cells are more therapeutic than T cells expanded in vitro.
Antigens-Thy-1: an, Cell-Separation, Cells-Cultured, Combined-Modality-Therapy, Comparative-Study, Cyclophosphamide: tu, Cytotoxicity-Immunologic, CD8-Positive-T-Lymphocytes: im, tr, Immunotherapy-Adoptive, Lymphocytes-Tumor-Infiltrating: im, tr, Mice, Mice-Inbred-C57BL, Remission-Induction, Rhabdomyosarcoma: dt, im, pa, th, Soft-Tissue-Neoplasms: dt, im, pa, th, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocyte-Subsets: im, tr
Anticancer Res 1995 Mar-Apr;15(2):441-7
CA27523/CA/NCI, CA34196/CA/NCI, CA57784/CA/NCI
Adoptive immunotherapy (AIT) involving transfer of tumor-sensitized T lymphocytes in combination with cyclophosphamide (CY)-injection results in the eradication of the C57BL/6J (B6) rhabdomyosarcoma, 76-9 and is associated with the accumulation of a large number of tumor-infiltrating lymphocytes (TIL). Using immune spleen cells (ISC) from B6 and congenic B6. PL. Thy-1a mice, it was shown that most (> or = 97%) of the TIL were donor-derived. This in situ increase in donor-derived T cells was confirmed by using positively-selected Thy- 1.1+ and Thy- 1.2+ TIL for AIT after isolating them from regressing tumors and expanding them in rIL-2. The extent of CD8+ TIL expansion in vivo correlated with the numbers of TIL adoptively transferred and this in turn determined the degree of anti-tumor effects. It was evident, however, that these in vitro-expanded TIL expressing mRNA for TNF alpha and IFN gamma were qualitatively different and therapeutically less efficacious than the T cells associated with ISC or with freshly-isolated TIL. Unlike freshly isolated TIL that expressed specific cytotoxicity towards the 76-9 targets in vitro, IL-2 expanded TIL killed 76-9 cells and unrelated tumor targets to the same extent. A cytotoxic CD8+ T cell line derived from ISC and selected for activity against the 76-9 tumor cells showed no therapeutic efficacy. The data suggest that, in this tumor model, expansion of CD8+ T cells in vitro selects against anti-tumor efficacy.
Evans, R; Kamdar, S J.; Duffy, T M.; Krupke, D M.; Fuller, J A.; and Dudley, M E., " The therapeutic efficacy of murine anti-tumor T cells: freshly isolated T cells are more therapeutic than T cells expanded in vitro." (1995). Faculty Research 1990 - 1999. 632.