Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation.
Nature 1995 Jul 6;376(6535):70-4
Tie-1 and Tie-2 define a new class of receptor tyrosine kinases that are specifically expressed in developing vascular endothelial cells. To study the functions of Tie-1 and Tie-2 during vascular endothelial cell growth and differentiation in vivo, targeted mutations of the genes in mice were introduced by homologous recombination. Embryos deficient in Tie-1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. However, analyses of embryos deficient in Tie-2 showed that it is important in angiogenesis, particularly for vascular network formation in endothelial cells. This result contrasts with previous reports on Tie-2 function in vasculogenesis and/or endothelial cell survival. Our in vivo analyses indicate that the structurally related receptor tyrosine kinases Tie-1 and Tie-2 have important but distinct roles in the formation of blood vessels.
Sato, T N.; Tozawa, Y; Deutsch, U; Wolburg, Buchholz K.; Fujiwara, Y; Gendron, Maguire M.; Gridley, T; Wolburg, H; Risau, W; and Qin, Y, " Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation." (1995). Faculty Research 1990 - 1999. 676.