Faculty Research 1990 - 1999

Function of untranslated regions in the mouse spermatogenesis-specific gene Tcp10 evaluated in transgenic mice.

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Gene-Expression, Male, Mice, Mice-Transgenic, Nuclear-Proteins: ge, Sequence-Analysis-DNA, Spermatogenesis: ge, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Testicular-Hormones: ge

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DNA Cell Biol 1997 May;16(5):645-51




The mouse Tcp10 genes are transcribed exclusively in male germ cells and display multiple 5' and 3' untranslated variations generated by alternative splicing and polyadenylation signal usage. To investigate the possible role of untranslated sequences in the regulation of these genes, chimeric expression constructs with or without endogenous 5' and 3' untranslated sequences were generated and used to make transgenic mice. Analysis of these animals showed that the untranslated sequences have no effect on the transcription or translation of an attached lacZ reporter gene, thereby implying these sequences are dispensible. However, the endogenous pattern of polyadenylation site usage was altered when Tcp10 3' untranslated sequences were linked to lacZ, indicating that internal coding sequence can influence recognition of polyadenylation signals in testis. The characteristics of alternative splicing and polyadenylation signal variability reflects a common theme of promiscuity in testicular gene expression.

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