A torrid zone on mouse chromosome 1 containing a cluster of recombinational hotspots.
Document Type
Article
Publication Date
2005
First Page
833
Last Page
841
JAX Source
Genetics 2005 Feb; 169(2):833-41.
Abstract
Within the 2.38-Mb Ath1 region of mouse chromosome 1, 42 of 45 genetic crossovers from crosses between C57BL/6J (B6) and either C3H/HeJ (H) or Mus spretus (SPRET) occurred in four zones (A-D); zone A, 100 kb long, contained a cluster of at least four recombination hotspots. F1 sperm assays indicate that within this "torrid zone" the most active hotspot (A3) can initiate recombination on H and SPRET but not B6 chromosomes. The A3 DNA sequence contains a (G/C)TTT repeat, long stretches of A or T, and a cyclic variation in AT content. Recombination was drastically reduced in a cross between B6 and a B6.SPRET Ath1 congenic strain, but was unaffected in a B6 x B6.H Ath1 congenic cross. Similar nonrandom clustering of hotspots has been observed in yeast and the major histocompatibility complexes of human and mouse. To the extent that torrid zones are a general feature of mammalian genomes, they have considerable implications for genetic mapping strategies in both human populations and mouse crosses.
Recommended Citation
Kelmenson PM,
Petkov P,
Wang X,
Higgins DC,
Paigen BJ,
Paigen K.
A torrid zone on mouse chromosome 1 containing a cluster of recombinational hotspots. Genetics 2005 Feb; 169(2):833-41.