In vitro fertilization with cryopreserved inbred mouse sperm.

Document Type


Publication Date



Blastocyst, Cryopreservation, Embryo-Transfer, Female, Fertilization-in-Vitro, Male, Mice, Mice-Inbred-Strains, Ovum, Pregnancy, Semen-Preservation, Species-Specificity, Sperm-Motility, Spermatozoa, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Biol Reprod 2000 Dec; 63(6):1774-80.


RR01262/RR/NCRR, RR09781/RR/NCRR, RR15012/RR/NCRR


Sperm from C57BL/6J, DBA/2J, BALB/cJ, 129S3/SvImJ, and FVB/NJ inbred mice were cryopreserved in 3% skim milk/18% raffinose cryoprotectant solution. The post-thaw sperm from all strains were evaluated for their viability and fertility by comparing them against B6D2F1 sperm used as a control. The protocol used for freezing mouse sperm was effective in different strains, because the motility was decreased by 50% after cryopreservation similar to other mammalian sperm. However, the progressive motility and the fertility of each inbred strain were affected differently. The C57BL/6J, BALB/cJ, and 129S3/SvImJ strains were the most affected; their fertility (two-cell cleavage) decreased from 70%, 34%, and 84% when using freshly collected sperm to 6%, 12%, and 6% when using frozen/thawed sperm, respectively. Live newborns derived from frozen/thawed sperm were obtained from all strains in the study. These results corroborate the genetic variation among strains with regard to fertility and susceptibility to cryopreservation.

Please contact the Joan Staats Library for information regarding this document.