QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains.

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Animals, Cholesterol, Chromosome-Mapping, Chromosomes-Mammalian, Crosses-Genetic, Female, Genotype, Haplotypes, Linkage-(Genetics), Lipoproteins, Lipoproteins-HDL-Cholesterol, Male, Mice, Mice-Inbred-DBA, Mice-Inbred-Strains, Phenotype, Quantitative-Trait-Loci, Regression-Analysis, Sequence-Analysis-DNA

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J Lipid Res 2006 Aug; 47(8):1780-90.


To identify additional loci that influence lipoprotein cholesterol levels, we performed quantitative trait locus (QTL) mapping in offspring of PERA/EiJxI/LnJ and PERA/EiJxDBA/2J intercrosses and in a combined data set from both crosses after 8 weeks of consumption of a high fat-diet. Most QTLs identified were concordant with homologous chromosomal regions that were associated with lipoprotein levels in human studies. We detected significant new loci for HDL cholesterol levels on chromosome (Chr) 5 (Hdlq34) and for non-HDL cholesterol levels on Chrs 15 (Nhdlq9) and 16 (Nhdlq10). In addition, the analysis of combined data sets identified a QTL for HDL cholesterol on Chr 17 that was shared between both crosses; lower HDL cholesterol levels were conferred by strain PERA. This QTL colocalized with a shared QTL for cholesterol gallstone formation detected in the same crosses. Haplotype analysis narrowed this QTL, and sequencing of the candidate genes Abcg5 and Abcg8 confirmed shared alleles in strains I/LnJ and DBA/2J that differed from the alleles in strain PERA/EiJ. In conclusion, our analysis furthers the knowledge of genetic determinants of lipoprotein cholesterol levels in inbred mice and substantiates the hypothesis that polymorphisms of Abcg5/Abcg8 contribute to individual variation in both plasma HDL cholesterol levels and susceptibility to cholesterol gallstone formation.