Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus.
Antibodies-Viral, Genetic-Vectors, Macaca-mulatta, SAIDS-Vaccines, Simian-Acquired-Immunodeficiency-Syndrome, Simian-immunodeficiency-virus, Simplexvirus, Vaccination, Vaccines-DNA
Virology 2007 Jan; 357(2):199-214.
The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three other macaques were primed with recombinant DNA vectors expressing Gag, Env, and a Pol-Tat-Nef-Vif fusion protein prior to boosting with the HSV vectors. Robust anti-Gag and anti-Env cellular responses were detected in all six macaques. Following intravenous challenge with wild-type, cloned SIV239, peak and 12-week plasma viremia levels were significantly lower in vaccinated compared to control macaques. Plasma SIV RNA in vaccinated macaques was inversely correlated with anti-Rev ELISPOT responses on the day of challenge (P value<0.05), anti-Tat ELISPOT responses at 2 weeks post challenge (P value <0.05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS.
Kaur, A; Sanford, H B.; Garry, D; Lang, S; Klumpp, S A.; Watanabe, D; Bronson, R T.; Lifson, J D.; Rosati, M; Pavlakis, G N.; Felber, B K.; Knipe, D M.; and Desrosiers, R C., "Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus." (2007). Faculty Research 2000 - 2009. 1492.