Title
Alu elements mediate MYB gene tandem duplication in human T-ALL.
Document Type
Article
Publication Date
2007
Keywords
Base-Sequence, Gene-Duplication, Humans, In-Situ-Hybridization-Fluorescence, Leukemia-Lymphoma-Adult-T-Cell, Models-Biological, Models-Genetic, Molecular-Sequence-Data, Nucleic-Acid-Hybridization, Polymerase-Chain-Reaction, Proto-Oncogene-Proteins-c-myb, Recombination-Genetic
First Page
3059
Last Page
3066
JAX Source
J Exp Med 2007 Dec; 204(13):3059-66.
Abstract
Recent studies have demonstrated that the MYB oncogene is frequently duplicated in human T cell acute lymphoblastic leukemia (T-ALL). We find that the human MYB locus is flanked by 257-bp Alu repeats and that the duplication is mediated somatically by homologous recombination between the flanking Alu elements on sister chromatids. Nested long-range PCR analysis indicated a low frequency of homologous recombination leading to MYB tandem duplication in the peripheral blood mononuclear cells of approximately 50% of healthy individuals, none of whom had a MYB duplication in the germline. We conclude that Alu-mediated MYB tandem duplication occurs at low frequency during normal thymocyte development and is clonally selected during the molecular pathogenesis of human T-ALL.
Recommended Citation
O'Neil J,
Tchinda J,
Gutierrez A,
Moreau L,
Maser RS,
Wong KK,
Li W,
McKenna K,
Liu XS,
Feng B,
Neuberg D,
et a.
Alu elements mediate MYB gene tandem duplication in human T-ALL. J Exp Med 2007 Dec; 204(13):3059-66.