Novel Sxr(a) ES cell line offers hope for Y chromosome gene-targeted mice.
Genesis 2002 Jun; 33(2):62-6.
1R01MH/HD57465/MH/NIMH, CA34196/CA/NCI, HG00098/HG/NHGRI
A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XYSxr(a) mouse, which carries a duplication of the gene-rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the YSxr(a) chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes.
Simpson, E M.; Johnson, K A.; Shirley, B J.; Fang, G Y.; Bayleran, J K.; and Lerner, C P., " Novel Sxr(a) ES cell line offers hope for Y chromosome gene-targeted mice." (2002). Faculty Research 2000 - 2009. 396.