Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates.
Document Type
Article
Publication Date
2002
Keywords
Bone-and-Bones, Cells-Cultured, Chondrocytes, Embryonic-Induction, Female, Growth-Disorders, Growth-Plate, Human, Humerus, In-Situ-Hybridization, Male, Membrane-Proteins, Mice, Mice-Inbred-C57BL, Mice-Knockout, Osteoblasts, Proliferating-Cell-Nuclear-Antigen, Receptors-Cell-Surface, Repressor-Proteins, Signal-Transduction, Skull, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tibia, Trans-Activators
First Page
79
Last Page
89
JAX Source
Dev Dyn 2002 May; 224(1):79-89.
Grant
AR36819/AR/NIAMS, AR36820/AR/NIAMS, HD34883/HD/NICHD, NS36437/NS/NINDS
Abstract
Gene-targeted disruption of Grg5, a mouse homologue of Drosophila groucho (gro), results in postnatal growth retardation in mice. The growth defect, most striking in approximately half of the Grg5 null mice, occurs during the first 4-5 weeks of age, but most mice recover retarded growth later. We used the nonlinear mixed-effects model to fit the growth data of wild-type, heterozygous, and Grg5 null mice. On the basis of preliminary evidence suggesting an interaction between Grg5 and the transcription factor Cbfa1/Runx2, critical for skeletal development, we further investigated the skeleton in the mice. A long bone growth plate defect was identified, which included shorter zones of proliferative and hypertrophic chondrocytes and decreased trabecular bone formation. This decreased trabecular bone formation is likely caused by a reduced recruitment of osteoblasts into the growth plate region of Grg5 null mice. Like the growth defect, the growth plate and trabecular bone abnormality improved as the mice grew older. The growth plate defect was associated with reduced Indian hedgehog expression and signaling. We suggest that Grg5, a transcriptional coregulator, modulates the activities of transcription factors, such as Cbfa1/Runx2 in vivo to affect Ihh expression and the function of long bone growth plates.
Recommended Citation
Wang WF,
Wang YG,
Reginato AM,
Plotkina S,
Gridley T,
Olsen BR.
Growth defect in Grg5 null mice is associated with reduced Ihh signaling in growth plates. Dev Dyn 2002 May; 224(1):79-89.