beta2 microglobulin-deficient (B2m(null)) NOD/SCID mice are excellent recipients for studying human stem cell function.

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Animal, Cell-Differentiation, Cell-Division, Disease-Models-Animal, Gene-Deletion, Hematopoiesis, Hematopoietic-Stem-Cell-Transplantation, Hematopoietic-Stem-Cells, Human, Mice, Mice-Inbred-NOD, Mice-SCID, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

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Blood 2000 May; 95(10):3102-5.


A130389, DK57199/DK/NIDDK


Human SCID repopulating cells (SRC) are defined based on their functional ability to repopulate the bone marrow of NOD/SCID mice with both myeloid and lymphoid cell populations. The frequency of SRC in umbilical cord blood cells is 1 in 9.3 x 10(5) mononuclear cells. We report that as few as 8 x 10(4) human cord blood mononuclear cells transplanted into NOD/SCID/B2m(null )mice resulted in multilineage differentiation in the murine bone marrow, revealing a more than 11-fold higher SRC frequency than in NOD/SCID mice. Moreover, as few as 2 to 5 x 10(3) CD34(+) cells recovered from the bone marrow of primary transplanted NOD/SCID mice were sufficient for engrafting secondary NOD/SCID/B2m(null )mice with SRC, suggesting SRC self-renewal. Thus, by using NOD/SCID/B2m(null )mice as recipients, we established a functional assay for human stem cells capable of engrafting the bone marrow of primary and secondary transplanted immune-deficient mice with SRC, providing a model that better resembles autologous stem cell transplantation.

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