Systems biology of the 2-cell mouse embryo.
Cytogenet Genome Res 2004; 105(2-4):240-50.
The transcriptome of the 2-cell mouse embryo was analyzed to provide insight into the molecular networks at play during nuclear reprogramming and embryonic genome activation. Analysis of ESTs from a 2-cell cDNA library identified nearly 4,000 genes, over half of which have not been previously studied. Transcripts of mobile elements, especially those of LTR retrotransposons, are abundantly represented in 2-cell embryos, suggesting their possible role in introducing genomic variation, and epigenetic restructuring of the embryonic genome. Analysis of Gene Ontology of the 2-cell-stage expressed genes outlines the major biological processes that guide the oocyte-to-embryo transition. These results provide a foundation for understanding molecular control at the onset of mammalian development.
Evsikov, A V.; de, Vries W.; Peaston, A E.; Radford, E E.; Fancher, K S.; Chen, F H.; Blake, J A.; Bult, C J.; Latham, K E.; Solter, D; and Knowles, B B., " Systems biology of the 2-cell mouse embryo." (2004). Faculty Research 2000 - 2009. 770.