Transgenic Cre expression mice for generation of erythroid-specific gene alterations.
Genesis 2004 May; 39(1):1-9.
Transgenic mice that express Cre recombinase in erythroid cell lineages were developed so that genes affecting erythropoiesis/hematopoiesis may be altered without necessarily affecting fetus viability. A micro-LCR cassette-beta-globin promoter-Cre recombinase gene (microLCR-betapr-Cre) construct was synthesized and used to generate transgenic mice. Concurrently, we produced mice containing a microLCR-loxP-flanked beta sickle gene (microLCR-loxP-beta(S)-loxP) construct. microLCR-betapr-Cre mice with intact transgenes in variable copy number were identified. Cre expression was assessed by RNAse protection and RT-PCR. Cre function was ascertained by breeding to microLCR-loxP-beta(S)-loxP mice. We demonstrate that beta(S) expression was not detected in the blood of bigenics, but the gene was present in nonerythroid cells. Thus, excision of the loxP-flanked beta(S) gene was restricted to erythroid cell lineages.
Peterson, K R.; Fedosyuk, H; Zelenchuk, L; Nakamoto, B; Yannaki, E; Stamatoyannopoulos, G; Ciciotte, S; Peters, L L.; Scott, L M.; and Papayannopoulou, T, " Transgenic Cre expression mice for generation of erythroid-specific gene alterations." (2004). Faculty Research 2000 - 2009. 784.