Snail regulates p21(WAF/CIP1) expression in cooperation with E2A and Twist.
Cell-Differentiation, Cell-Line, Cells-Cultured, DNA-Binding-Proteins, Fibroblasts, Gene-Expression-Regulation, Humans, Nuclear-Proteins, Osteoblasts, Recombinant-Proteins, Repressor-Proteins, Transcription-Factors
Biochem Biophys Res Commun 2004 Dec; 325(4):1136-44.
Snail, a zinc-finger transcriptional repressor, is essential for mesoderm and neural crest cell formation and epithelial-mesenchymal transition. The basic helix-loop-helix transcription factors E2A and Twist have been linked with Snail during embryonic development. In this study, we examined the role of Snail in cellular differentiation through regulation of p21(WAF/CIP1) expression. A reporter assay with the p21 promoter demonstrated that Snail inhibited expression of p21 induced by E2A. Co-expression of Snail with Twist showed additive inhibitory effects. Deletion mutants of the p21 promoter revealed that sequences between -270 and -264, which formed a complex with unidentified nuclear factor(s), were critical for E2A and Snail function. The E2A-dependent expression of the endogenous p21 gene was also inhibited by Snail.
Takahashi, E; Funato, N; Higashihori, N; Hata, Y; Gridley, T; and Nakamura, M, "Snail regulates p21(WAF/CIP1) expression in cooperation with E2A and Twist." (2004). Faculty Research 2000 - 2009. 979.