In vivo selection of human embryonic stem cell-derived cells expressing methotrexate-resistant dihydrofolate reductase.
Bone-Marrow, Cell-Differentiation, Cell-Line, Drug-Resistance, Embryonic-Stem-Cells, Gene-Therapy, Graft-Survival, Humans, Methotrexate, Mice-Inbred-NOD, Mice-SCID, Stem-Cell-Transplantation, Teratoma, Tetrahydrofolate-Dehydrogenase
see Reprint Collection (a pdf is available).
Gene Ther 2010 Feb; 17(2):238-49.
Human embryonic stem cells (hESCs) provide a novel source of hematopoietic and other cell populations suitable for gene therapy applications. Preclinical studies to evaluate engraftment of hESC-derived hematopoietic cells transplanted into immunodeficient mice demonstrate only limited repopulation. Expression of a drug-resistance gene, such as Tyr22-dihydrofolate reductase (Tyr22-DHFR), coupled to methotrexate (MTX) chemotherapy has the potential to selectively increase the engraftment of gene-modified, hESC-derived cells in mouse xenografts. Here, we describe the generation of Tyr22-DHFR-GFP-expressing hESCs that maintain pluripotency, produce teratomas and can differentiate into MTXr-hemato-endothelial cells. We demonstrate that MTX administered to nonobese diabetic/severe combined immunodeficient/IL-2Rgammac(null) (NSG) mice after injection of Tyr22-DHFR-hESC-derived cells significantly increases human CD34(+) and CD45(+) cell engraftment in the bone marrow (BM) and peripheral blood of transplanted MTX-treated mice. These results demonstrate that MTX treatment supports selective, long-term engraftment of Tyr22-DHFR cells in vivo, and provides a novel approach for combined human cell and gene therapy.
Gori, J L.; Tian, X; Swanson, D; Gunther, R; Shultz, L D.; McIvor, R S.; and Kaufman, D S., "In vivo selection of human embryonic stem cell-derived cells expressing methotrexate-resistant dihydrofolate reductase." (2010). Faculty Research 2010. 30.