ZFP191 is required by oligodendrocytes for CNS myelination.
Animals, Carrier-Proteins, Cell-Differentiation, Cell-Line, Cell-Proliferation, Cells-Cultured, Central-Nervous-System, Embryo-Mammalian, Mice-Transgenic, Mutation, Myelin-Sheath, Oligodendroglia
Genes Dev 2010 Feb; 24(3):301-11.
The controlling factors that prompt mature oligodendrocytes to myelinate axons are largely undetermined. In this study, we used a forward genetics approach to identify a mutant mouse strain characterized by the absence of CNS myelin despite the presence of abundant numbers of late-stage, process-extending oligodendrocytes. Through linkage mapping and complementation testing, we identified the mutation as a single nucleotide insertion in the gene encoding zinc finger protein 191 (Zfp191), which is a widely expressed, nuclear-localized protein that belongs to a family whose members contain both DNA-binding zinc finger domains and protein-protein-interacting SCAN domains. Zfp191 mutants express an array of myelin-related genes at significantly reduced levels, and our in vitro and in vivo data indicate that mutant ZFP191 acts in a cell-autonomous fashion to disrupt oligodendrocyte function. Therefore, this study demonstrates that ZFP191 is required for the myelinating function of differentiated oligodendrocytes.
Howng, S Y.; Avila, R L.; Emery, B; Traka, M; Lin, W; Watkins, T; Cook, S; Bronson, R; Davisson, M; Barres, B A.; and Popko, B, "ZFP191 is required by oligodendrocytes for CNS myelination." (2010). Faculty Research 2010. 35.