Title

Structured illumination microscopy of autofluorescent aggregations in human tissue.

Document Type

Article

Publication Date

6-2011

Keywords

Structured illumination; Microscopy; Autofluorescence; Retinal pigment epithelium; Interferometer

JAX Source

Micron 2011 Jun; 42(4):330-335.

PMID

20926302

Abstract

Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647nm), thus enabling us to gather spectral information about the autofluorescence signal.

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