Title

Imaging label-free intracellular structures by localisation microscopy.

Document Type

Article

Publication Date

6-2011

Keywords

Sub-diffraction limit microscopy; Localisation microscopy; Spectral Precision Distance Microscopy (SPDM); Fluorescence

JAX Source

Micron 2011 Jun; 42(4):348-52.

PMID

20538472

Abstract

Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200nm laterally and 600nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the lambda/10 range ( approximately 50nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions.

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