Differentiating inbred mouse strains from each other and those with single gene mutations using hair proteomics.
Document Type
Article
Publication Date
2012
JAX Source
PLoS One 2012; 7(12):e51956.
PMID
23251662
Volume
7
Issue
12
First Page
51956
Last Page
51956
ISSN
1932-6203
Abstract
Mutant laboratory mice with distinctive hair phenotypes are useful for identifying genes responsible for hair diseases. The work presented here demonstrates that shotgun proteomic profiling can distinguish hair shafts from different inbred mouse strains. For this purpose, analyzing the total hair shaft provided better discrimination than analyzing the isolated solubilized and particulate (cross-linked) fractions. Over 100 proteins exhibited significant differences among the 11 strains and 5 mutant stocks across the wide spectrum of strains surveyed. Effects on the profile of single gene mutations causing hair shaft defects were profound. Since the hair shaft provides a discrete sampling of the species proteome, with constituents serving important functions in epidermal appendages and throughout the body, this work provides a foundation for non-invasive diagnosis of genetic diseases of hair and perhaps other tissues.
Recommended Citation
Rice R,
Bradshaw K,
Durbin-Johnson B,
Rocke D,
Eigenheer R,
Phinney B,
Sundberg JP.
Differentiating inbred mouse strains from each other and those with single gene mutations using hair proteomics. PLoS One 2012; 7(12):e51956.