Title
Choline kinase beta is required for normal endochondral bone formation.
Document Type
Article
Publication Date
3-14-2014
JAX Source
Biochim Biophys Acta 2014 Mar 14; 1840(7):2112-2122.
PMID
24637075
Abstract
BACKGROUND: Choline kinase has three isoforms encoded by the genes Chka and Chkb. Inactivation of Chka in mice results in embryonic lethality, whereas Chkb(-/-) mice display neonatal forelimb bone deformations.
METHODS: To understand the mechanisms underlying the bone deformations, we compared the biology and biochemistry of bone formation from embryonic to young adult wild-type (WT) and Chkb(-/-) mice.
RESULTS: The deformations are specific to the radius and ulna during the late embryonic stage. The radius and ulna of Chkb(-/-) mice display expanded hypertrophic zones, unorganized proliferative columns in their growth plates, and delayed formation of primary ossification centers. The differentiation of chondrocytes of Chkb(-/-) mice was impaired, as was chondrocyte proliferation and expression of matrix metalloproteinases 9 and 13. In chondrocytes from Chkb(-/-) mice, phosphatidylcholine was slightly lower than in WT mice whereas the amount of phosphocholine was decreased by approximately 75%. In addition, the radius and ulna from Chkb(-/-) mice contained fewer osteoclasts along the cartilage/bone interface.
CONCLUSIONS: Chkb has a critical role in the normal embryogenic formation of the radius and ulna in mice.
GENERAL SIGNIFICANCE: Our data indicate that choline kinase beta plays an important role in endochondral bone formation by modulating growth plate physiology.
Biochim Biophys Acta 2014 Mar 14; 1840(7):2112-2122.
Recommended Citation
Li, Zhuo; Wu, Gengshu; Sher, Roger B; Khavandgar, Zohreh; Hermansson, Martin; Cox, Gregory A.; Doschak, Michael R; Murshed, Monzur; Beier, Frank; and Vance, Dennis E, "Choline kinase beta is required for normal endochondral bone formation." (2014). Faculty Research 2014. 82.
https://mouseion.jax.org/stfb2014/82