Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation.
Document Type
Article
Publication Date
5-20-2016
JAX Source
J Genet Genomics 2016 May 20; 43(5):319-27
Volume
43
Issue
5
First Page
319
Last Page
327
ISSN
1673-8527
PMID
27210041
Grant
CA034196
Abstract
Previously we established Zygote Electroporation of Nucleases (ZEN) technology as an efficient and high-throughput way to generate genetically modified mouse models. However, there were significant variations of the targeting efficiency among different genomic loci using our previously published protocol. In this study, we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of electroporation. Using this approach, we were able to introduce precise nucleotide substitutions, large segment deletion and short segment insertion into targeted loci with high efficiency. J Genet Genomics 2016 May 20; 43(5):319-27
Recommended Citation
Wang W,
Kutny P,
Byers SL,
Longstaff C,
DaCosta M,
Pang C,
Zhang Y,
Taft RA,
Buaas FW,
Wang H.
Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation. J Genet Genomics 2016 May 20; 43(5):319-27