Ultra-High Resolution 3D Imaging of Whole Cells.
Document Type
Article
Publication Date
8-11-2016
JAX Source
Cell 2016 Aug 11; 166(4):1028-40
Volume
166
Issue
4
First Page
1028
Last Page
1040
ISSN
1097-4172
PMID
27397506
Grant
GM99640
Abstract
Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50-80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes. Cell 2016 Aug 11; 166(4):1028-40.
Recommended Citation
Huang F,
Sirinakis G,
Allgeyer E,
Schroeder L,
Duim W,
Kromann E,
Phan T,
Rivera-Molina F,
Myers J,
Irnov I,
Lessard M,
Zhang Y,
Handel M,
Jacobs-Wagner C,
Lusk C,
Rothman J,
Toomre D,
Booth M,
Bewersdorf J.
Ultra-High Resolution 3D Imaging of Whole Cells. Cell 2016 Aug 11; 166(4):1028-40