Generation of Genetically Modified Mice Using the CRISPR-Cas9 Genome-Editing System.
Cold Spring Harb Protoc 2016 Feb 1; 2016(2):150-9.
Genetically modified mice are extremely valuable tools for studying gene function and human diseases. Although the generation of mice with specific genetic modifications through traditional methods using homologous recombination in embryonic stem cells has been invaluable in the last two decades, it is an extremely costly, time-consuming, and, in some cases, uncertain technology. The recently described CRISPR-Cas9 genome-editing technology significantly reduces the time and the cost that are required to generate genetically engineered mice, allowing scientists to test more precise and bold hypotheses in vivo. Using this revolutionary methodology we have generated more than 100 novel genetically engineered mouse strains. In the current protocol, we describe in detail the optimal conditions to generate mice carrying point mutations, chromosomal deletions, conditional alleles, fusion tags, or endogenous reporters. Cold Spring Harb Protoc 2016 Feb 1; 2016(2):150-9.
Henao-Mejia, Jorge; Williams, Adam; Rongvaux, Anthony; Stein, Judith; Hughes, Cynthia; and Flavell, Richard A, "Generation of Genetically Modified Mice Using the CRISPR-Cas9 Genome-Editing System." (2016). Faculty Research 2016. 34.