Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing.

Authors

Wenning Qin, The Jackson LaboratoryFollow
Peter Kutny, The Jackson LaboratoryFollow
Richard S. Maser, The Jackson LaboratoryFollow
Stephanie L. Dion, The Jackson LaboratoryFollow
Jeffrey D Lamont, The Jackson LaboratoryFollow
Yingfan Zhang, The Jackson LaboratoryFollow
Greggory A Perry, The Jackson LaboratoryFollow
Haoyi Wang, The Jackson LaboratoryFollow

Document Type

Article

Publication Date

3-1-2016

JAX Source

Curr Protoc Mouse Biol 2016 Mar 1; 6(1):39-66.

Volume

6

Issue

1

First Page

39

Last Page

66

ISSN

2161-2617

PMID

26928663

Abstract

The CRISPR-Cas9 system in bacteria and archaea has recently been exploited for genome editing in various model organisms, including mice. The CRISPR-Cas9 reagents can be delivered directly into the mouse zygote to derive a mutant animal carrying targeted genetic modifications. The major components of the system include the guide RNA, which provides target specificity, the Cas9 nuclease that creates the DNA double-strand break, and the donor oligonucleotide or plasmid carrying the intended mutation flanked by sequences homologous to the target site. Here we describe the general considerations and experimental protocols for creating genetically modified mice using the CRISPR-Cas9 system. © 2016 by John Wiley & Sons, Inc. Curr Protoc Mouse Biol 2016 Mar 1; 6(1):39-66.

Recommended Citation

Qin W, Kutny P, Maser RS, Dion SL, Lamont J, Zhang Y, Perry G, Wang H. Generating Mouse Models Using CRISPR-Cas9-Mediated Genome Editing. Curr Protoc Mouse Biol 2016 Mar 1; 6(1):39-66.