Phenotyping first-generation genome editing mutants: a new standard?
Mamm Genome 2017 Aug; 28(7-8):377-382.
The unprecedented efficiency of the CRISPR/Cas9 system in genome engineering has opened the prospect of employing mutant founders for phenotyping cohorts, thus accelerating research projects by circumventing the requirement to generate cohorts using conventional two- or three-generation crosses. However, these first-generation mutants are often genetic mosaics, with a complex and difficult to define genetic make-up. Here, we discuss the potential benefits, challenges and scientific validity of such models. Mamm Genome 2017 Aug; 28(7-8):377-382.
Teboul, Lydia; Murray, Stephen A.; and Nolan, Patrick M, "Phenotyping first-generation genome editing mutants: a new standard?" (2017). Faculty Research 2017. 181.