Title

Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing.

Document Type

Article

Publication Date

9-1-2018

JAX Source

J Immunol 2018 Sep 1; 201(5):1586-1598

PMID

30021769

DOI

https://doi.org/10.4049/jimmunol.1701616

Grant

AI121920, NS105539

Abstract

Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (

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