Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing.
J Immunol 2018 Sep 1; 201(5):1586-1598
Developing precise and efficient gene editing approaches using CRISPR in primary human T cell subsets would provide an effective tool in decoding their functions. Toward this goal, we used lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the Cas9 gene and guide RNAs that targeted either coding or noncoding regions of genes of interest. We showed that multiple genes (
Chen, Xin; Kozhaya, Lina; Tastan, Cihan; Placek, Lindsey; Dogan, Mikail; Horne, Meghan; Abblett, Rebecca; Karhan, Ece; Vaeth, Martin; Feske, Stefan; and Unutmaz, Derya, "Functional Interrogation of Primary Human T Cells via CRISPR Genetic Editing." (2018). Faculty Research 2018. 165.