JMG, JGM, JAXCA
Sci Rep 2018 Dec 18; 8(1):17937
CA034196,CA191848,CA224067,CA230031, Hope Foundation
The processes by which tumors evolve are essential to the efficacy of treatment, but quantitative understanding of intratumoral dynamics has been limited. Although intratumoral heterogeneity is common, quantification of evolution is difficult from clinical samples because treatment replicates cannot be performed and because matched serial samples are infrequently available. To circumvent these problems we derived and assayed large sets of human triple-negative breast cancer xenografts and cell cultures from two patients, including 86 xenografts from cyclophosphamide, doxorubicin, cisplatin, docetaxel, or vehicle treatment cohorts as well as 45 related cell cultures. We assayed these samples via exome-seq and/or high-resolution droplet digital PCR, allowing us to distinguish complex therapy-induced selection and drift processes among endogenous cancer subclones with cellularity uncertainty
Kim, Hyunsoo; Kumar, Pooja A; Menghi, Francesca; Noorbakhsh, Javad; Cerveira, Eliza; Ryan, Mallory; Zhu, Qihui; Ananda, Guruprasad; George, Joshy; Chen, Henry C; Mockus, Susan; Zhang, Chengsheng; Yang, Yan; Keck, James G.; Karuturi, Radha Krishna Murthy; Bult, Carol J; Lee, Charles; Liu, Edison; and Chuang, Jeffrey H, "High-resolution deconstruction of evolution induced by chemotherapy treatments in breast cancer xenografts." (2018). Faculty Research 2018. 245.