Title

Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation.

Document Type

Article

Publication Date

1-2019

JAX Location

Reprint Collection

JAX Source

Methods Mol Biol 2019; 1874:179-190

PMID

30353514

DOI

https://doi.org/10.1007/978-1-4939-8831-0_10

Grant

CA034196

Abstract

The CRISPR-Cas9 system in bacteria and archaea has recently been exploited for genome editing in various model organisms, including the mice. In this scheme, components of the CRISPR-Cas9 system are delivered into the mouse zygote and mutant mice carrying genetic modifications derived. Although microinjection has been the technology of choice, electroporation has also emerged and been proven to be effective delivering CRISPR-Cas9 reagents into the mouse zygote. Here, we describe the experimental protocol employing electroporation to deliver CRISPR-Cas9 reagents into mouse embryos and derive gene-edited mutant mice.

Comments

We would like to thank the Genetic Engineering Technologies and the Reproductive sciences groups of the Jackson Laboratory for their partnership and contribution exploring into the CRISPR-Cas9 technology.

Please contact the Joan Staats Library for information regarding this document.

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