Title

Longitudinal profiling of human blood transcriptome in healthy and lupus pregnancy.

Document Type

Article

Publication Date

5-2019

Keywords

JGM

JAX Source

J Exp Med 2019 May; 216(5):1154-1169

PMID

30962246

DOI

https://doi.org/10.1084/jem.20190185

Grant

AR070594

Abstract

Systemic lupus erythematosus carries an increased risk of pregnancy complications, including preeclampsia and fetal adverse outcomes. To identify the underlying molecular mechanisms, we longitudinally profiled the blood transcriptome of 92 lupus patients and 43 healthy women during pregnancy and postpartum and performed multicolor flow cytometry in a subset of them. We also profiled 25 healthy women undergoing assisted reproductive technology to monitor transcriptional changes around embryo implantation. Sustained down-regulation of multiple immune signatures, including interferon and plasma cells, was observed during healthy pregnancy. These changes appeared early after embryo implantation and were mirrored in uncomplicated lupus pregnancies. Patients with preeclampsia displayed early up-regulation of neutrophil signatures that correlated with expansion of immature neutrophils. Lupus pregnancies with fetal complications carried the highest interferon and plasma cell signatures as well as activated CD4

Comments

Partially funded by the Kathryn W. Davis gift to The Jackson Laboratory.

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