Genetics 2019 Nov; 213(3):923-939
Vasa homologs are ATP-dependent DEAD-box helicases, multipotency factors, and critical components that specify and protect the germline. They regulate translation, amplify piwi-interacting RNAs (piRNAs), and act as RNA solvents; however, the limited availability of mutagenesis-derived alleles and their wide range of phenotypes have complicated their analysis. Now, with clustered regularly interspaced short palindromic repeats (CRISPR/Cas9), these limitations can be mitigated to determine why protein domains have been lost or retained throughout evolution. Here, we define the functional motifs of GLH-1/Vasa in
Marnik, Elisabeth A; Fuqua, J Heath; Sharp, Catherine S; Rochester, Jesse D; Xu, Emily L; Holbrook, Sarah E; and Updike, Dustin L, "Germline Maintenance Through the Multifaceted Activities of GLH/Vasa in" (2019). Faculty Research 2019. 249.