Document Type

Article

Publication Date

11-2019

Keywords

JMG

JAX Source

Genetics 2019 Nov; 213(3):923-939

Volume

213

Issue

3

First Page

923

Last Page

939

ISSN

1943-2631

PMID

31506335

DOI

https://doi.org/10.1534/genetics.119.302670

Abstract

Vasa homologs are ATP-dependent DEAD-box helicases, multipotency factors, and critical components that specify and protect the germline. They regulate translation, amplify piwi-interacting RNAs (piRNAs), and act as RNA solvents; however, the limited availability of mutagenesis-derived alleles and their wide range of phenotypes have complicated their analysis. Now, with clustered regularly interspaced short palindromic repeats (CRISPR/Cas9), these limitations can be mitigated to determine why protein domains have been lost or retained throughout evolution. Here, we define the functional motifs of GLH-1/Vasa in

Comments

We would like to thank Chris Smith in the Mount Desert Island Biological Laboratory (MDIBL) Sequencing Core for her assistance in sequencing and genotyping strains generated for this publication; Dorothy Wheatcraft at the mass spectrometry facility at the Jackson Laboratory for proteomics assistance and advice; Matt Stokes at Cell Signaling Technologies, Inc. for performing the proteomics and analysis of our GLH-1 IPs; Jarod Rollins at the MDIBL for ribosome antibodies.

Available freely online through the author-supported open access option.

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