Document Type

Article

Publication Date

2-18-2019

Keywords

JMG

JAX Source

Commun Biol 2019 Feb 18; 2:70

PMID

30793048

DOI

https://doi.org/10.1038/s42003-019-0321-x

Abstract

A persistent concern with CRISPR-Cas9 gene editing has been the potential to generate mutations at off-target genomic sites. While CRISPR-engineering mice to delete a ~360 bp intronic enhancer, here we discovered a founder line that had marked immune dysregulation caused by a 24 kb tandem duplication of the sequence adjacent to the on-target deletion. Our results suggest unintended repair of on-target genomic cuts can cause pathogenic "bystander" mutations that escape detection by routine targeted genotyping assays.

Comments

This open access article is licensed under a Creative Commons Attribution 4.0 International License

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